Publications
Google Scholar ProfilePrp8 in a Reduced Spliceosome Lacks a Conserved Toggle that Correlates with Splicing Complexity across Diverse Taxa.
Garside EL, Whelan TA, Stark MR, Rader SD, Fast NM, MacMillan AM.
J Mol Biol. 2019 Jun 28;431(14):2543-2553. doi: 10.1016/j.jmb.2019.04.047. Epub 2019 May 9.
Radical probing of spliceosome assembly.
Grewal CS, Kent OA, MacMillan AM.
Methods. 2017 Aug 1;125:16-24. doi: 10.1016/j.ymeth.2017.06.030. Epub 2017 Jun 29.
Silencing of natural transformation by an RNA chaperone and a multitarget small RNA.
Attaiech L, Boughammoura A, Brochier-Armanet C, Allatif O, Peillard-Fiorente F, Edwards RA, Omar AR, MacMillan AM, Glover M, Charpentier X.
Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):8813-8. doi: 10.1073/pnas.1601626113. Epub 2016 Jul 18.
Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae.
Black CS, Garside EL, MacMillan AM, Rader SD.
Protein Sci. 2016 Apr;25(4):911-6. doi: 10.1002/pro.2894. Epub 2016 Feb 25.
Analysis of CRISPR Pre-crRNA Cleavage.
Garside EL, MacMillan AM.
Methods Mol Biol. 2015;1311:35-46. doi: 10.1007/978-1-4939-2687-9_3.
It takes two to tangle: Prp24 and spliceosome assembly.
Pomeranz Krummel DA, MacMillan AM.
Nat Struct Mol Biol. 2014 Jun;21(6):503-4. doi: 10.1038/nsmb.2838.
Cas9 in close-up.
Garside EL, MacMillan AM.
Nat Biotechnol. 2014 Apr;32(4):338-40. doi: 10.1038/nbt.2872.
A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Schellenberg MJ, Wu T, Ritchie DB, Fica S, Staley JP, Atta KA, LaPointe P, MacMillan AM.
Nat Struct Mol Biol. 2013 Jun;20(6):728-34. doi: 10.1038/nsmb.2556. Epub 2013 May 19.
Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA endonucleases.
Garside EL, Schellenberg MJ, Gesner EM, Bonanno JB, Sauder JM, Burley SK, Almo SC, Mehta G, MacMillan AM.
RNA. 2012 Nov;18(11):2020-8. doi: 10.1261/rna.033100.112. Epub 2012 Sep 24.
Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis.
Chaulk SG, Thede GL, Kent OA, Xu Z, Gesner EM, Veldhoen RA, Khanna SK, Goping IS, MacMillan AM, Mendell JT, Young HS, Fahlman RP, Glover JN.
RNA Biol. 2011 Nov-Dec;8(6):1105-14. doi: 10.4161/rna.8.6.17410. Epub 2011 Nov 1.
Recognition and maturation of effector RNAs in a CRISPR interference pathway.
Gesner EM, Schellenberg MJ, Garside EL, George MM, Macmillan AM.
Nat Struct Mol Biol. 2011 Jun;18(6):688-92. doi: 10.1038/nsmb.2042. Epub 2011 May 15.
Structural model of the p14/SF3b155 · branch duplex complex.
Schellenberg MJ, Dul EL, MacMillan AM.
RNA. 2011 Jan;17(1):155-65. doi: 10.1261/rna.2224411. Epub 2010 Nov 9.
Context-dependent remodeling of structure in two large protein fragments.
Schellenberg MJ, Ritchie DB, Wu T, Markin CJ, Spyracopoulos L, MacMillan AM.
J Mol Biol. 2010 Oct 1;402(4):720-30. doi: 10.1016/j.jmb.2010.08.020. Epub 2010 Aug 14.
Spliceosome structure: piece by piece.
Ritchie DB, Schellenberg MJ, MacMillan AM.
Biochim Biophys Acta. 2009 Sep-Oct;1789(9-10):624-33. doi: 10.1016/j.bbagrm.2009.08.010. Epub 2009 Sep 3. Review.
Proteins specifically modified with a chemical nuclease as probes of RNA-protein interaction.
Kent OA, Macmillan AM.
Methods Mol Biol. 2008;488:191-200. doi: 10.1007/978-1-60327-475-3_13.
Structural elucidation of a PRP8 core domain from the heart of the spliceosome.
Ritchie DB, Schellenberg MJ, Gesner EM, Raithatha SA, Stuart DT, Macmillan AM.
Nat Struct Mol Biol. 2008 Nov;15(11):1199-205. doi: 10.1038/nsmb.1505. Epub 2008 Oct 2.
Effect of pH on aqueous phenylalanine studied using a 265-nm pulsed light-emitting diode.
Macmillan AM, McGuinness CD, Sagoo K, McLoskey D, Pickup JC, Birch DJ.
Ann N Y Acad Sci. 2008;1130:300-4. doi: 10.1196/annals.1430.056.
Toward single-metal-ion sensing by Förster resonance energy transfer.
Sutter JU, Macmillan AM, Birch DJ, Rolinski OJ.
Ann N Y Acad Sci. 2008;1130:62-7. doi: 10.1196/annals.1430.037. Review.
Pre-mRNA splicing: a complex picture in higher definition.
Schellenberg MJ, Ritchie DB, MacMillan AM.
Trends Biochem Sci. 2008 Jun;33(6):243-6. doi: 10.1016/j.tibs.2008.04.004. Epub 2008 May 9. Review.
Single molecule level detection of allophycocyanin by surface enhanced resonance Raman scattering.
McGuinness CD, Macmillan AM, Karolin J, Smith WE, Graham D, Pickup JC, Birch DJ.
Analyst. 2007 Jul;132(7):633-4. doi: 10.1039/b706409h. Epub 2007 May 23.
Synthesis of oligo-RNAs with photocaged adenosine 2'-hydroxyls.
Chaulk SG, MacMillan AM.
Nat Protoc. 2007;2(5):1052-8. doi: 10.1038/nprot.2007.154.
FRET analysis of in vivo dimerization by RNA-editing enzymes.
Chilibeck KA, Wu T, Liang C, Schellenberg MJ, Gesner EM, Lynch JM, MacMillan AM.
J Biol Chem. 2006 Jun 16;281(24):16530-5. doi: 10.1074/jbc.M511831200. Epub 2006 Apr 17.
Crystal structure of a core spliceosomal protein interface.
Schellenberg MJ, Edwards RA, Ritchie DB, Kent OA, Golas MM, Stark H, Lührmann R, Glover JN, MacMillan AM.
Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1266-71. doi: 10.1073/pnas.0508048103. Epub 2006 Jan 23.
Characterization of a U2AF-independent commitment complex (E') in the mammalian spliceosome assembly pathway.
Kent OA, Ritchie DB, Macmillan AM.
Mol Cell Biol. 2005 Jan;25(1):233-40. doi: 10.1128/MCB.25.1.233-240.2005.
RNAi: running interference for the cell.
Kent OA, MacMillan AM.
Org Biomol Chem. 2004 Jul 21;2(14):1957-61. doi: 10.1039/b404932m. Epub 2004 Jun 28. Review.
Structuring of the 3' splice site by U2AF65.
Kent OA, Reayi A, Foong L, Chilibeck KA, MacMillan AM.
J Biol Chem. 2003 Dec 12;278(50):50572-7. doi: 10.1074/jbc.M307976200. Epub 2003 Sep 23.
Adenosine to inosine editing by ADAR2 requires formation of a ternary complex on the GluR-B R/G site.
Jaikaran DC, Collins CH, MacMillan AM.
J Biol Chem. 2002 Oct 4;277(40):37624-9. doi: 10.1074/jbc.M204126200. Epub 2002 Aug 5.
Early organization of pre-mRNA during spliceosome assembly.
Kent OA, MacMillan AM.
Nat Struct Biol. 2002 Aug;9(8):576-81. doi: 10.1038/nsb822.
A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.
Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC.
EMBO J. 2001 Aug 15;20(16):4536-46. doi: 10.1093/emboj/20.16.4536.
Separation of Spliceosome Assembly from Catalysis with Caged pre-mRNA Substrates A.M.M. acknowledges support from the Natural Sciences and Engineering Research Council of Canada and the Alberta Heritage Foundation for Medical Research.
Chaulk SG, MacMillan AM.
Angew Chem Int Ed Engl. 2001 Jun 1;40(11):2149-2152.
Separation of Spliceosome Assembly from Catalysis with Caged pre-mRNA Substrates.
Chaulk SG, MacMillan AM.
Angew Chem Int Ed Engl. 2001 Jun 1;40(11):2149-2152. doi: 10.1002/1521-3773(20010601)40:11<2149::AID-ANIE2149>3.0.CO;2-Z.
Kinetic analysis of the M1 RNA folding pathway.
Kent O, Chaulk SG, MacMillan AM.
J Mol Biol. 2000 Dec 15;304(5):699-705. doi: 10.1006/jmbi.2000.4263.
Studies of RNA cleavage by photolysis of N-hydroxypyridine-2(1H)-thione. A new photochemical footprinting method.
Chaulk SG, Pezacki JP, MacMillan AM.
Biochemistry. 2000 Aug 29;39(34):10448-53. doi: 10.1021/bi0009136.
Template-directed interference footprinting for RNA based on inosine-specific cleavage.
Jaikaran DC, MacMillan AM.
Bioorg Med Chem Lett. 2000 Aug 7;10(15):1633-5. doi: 10.1016/s0960-894x(00)00266-3.
Kinetic Footprinting of an RNA-Folding Pathway Using Peroxynitrous Acid.
Chaulk SG, MacMillan AM.
Angew Chem Int Ed Engl. 2000 Feb;39(3):521-523. doi: 10.1002/(sici)1521-3773(20000204)39:3<521::aid-anie521>3.0.co;2-j.
Evidence for helical unwinding of an RNA substrate by the RNA enzyme RNase P: use of an interstrand disulfide crosslink in substrate.
Pomeranz Krummel DA, Kent O, MacMillan AM, Altman S.
J Mol Biol. 2000 Feb 4;295(5):1113-8. doi: 10.1006/jmbi.1999.3424.
Characterization of the Tetrahymena ribozyme folding pathway using the kinetic footprinting reagent peroxynitrous acid.
Chaulk SG, MacMillan AM.
Biochemistry. 2000 Jan 11;39(1):2-8. doi: 10.1021/bi992167e.
Caged RNA: photo-control of a ribozyme reaction.
Chaulk SG, MacMillan AM.
Nucleic Acids Res. 1998 Jul 1;26(13):3173-8. doi: 10.1093/nar/26.13.3173.
SC35-mediated reconstitution of splicing in U2AF-depleted nuclear extract.
MacMillan AM, McCaw PS, Crispino JD, Sharp PA.
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):133-6. doi: 10.1073/pnas.94.1.133.
Dynamic association of proteins with the pre-mRNA branch region.
MacMillan AM, Query CC, Allerson CR, Chen S, Verdine GL, Sharp PA.
Genes Dev. 1994 Dec 15;8(24):3008-20. doi: 10.1101/gad.8.24.3008.
Direct identification of the active-site nucleophile in a DNA (cytosine-5)-methyltransferase.
Chen L, MacMillan AM, Chang W, Ezaz-Nikpay K, Lane WS, Verdine GL.
Biochemistry. 1991 Nov 19;30(46):11018-25. doi: 10.1021/bi00110a002.